3D Imaging of Transparent Biological Specimens with Fourier Ptychographic Microscopy
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2019
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Swarthmore College. Dept. of Engineering
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en
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Full copyright to this work is retained by the student author. It may only be used for non-commercial, research, and educational purposes. All other uses are restricted.
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Abstract
Imaging biological samples comes with a unique set of challenges. One of those
challenges is accounting for the thickness of the samples, requiring us to use three-dimensional imaging techniques. Due to their low refractive index, light usually passes
thought these biological samples. This allows us to get information from the samples in
the form of light, through methods such as Fourier Ptychographic Microscopy (FPM) and
phase-contrast microscopy. This opens a world of possibilities for different model and
algorithm designs to tackle the problem of 3D imaging. In this work, we optimize over an
implementation of Fourier Ptychographic Microscopy for a multislice forward model in
order to obtain marginal gains in intensity and phase contrast that result in significant
improvements to the outcome intensity and phase images.